ABSTRACT
PURPOSE: To evaluate the effect of ischemic preconditioning on mortality, inflammatory mediators and oxidative stress after intestinal ischemia and reperfusion. METHODS: Male Wistar rats were allocated according to the period of ischemia with or without ischemic preconditioning which consist on clamping the superior mesenteric artery for 10 minutes followed by reperfusion for 10 minutes before the sustained ischemia period. Mortality was assessed in Phase 1 study, and the CINC-1, CINC-2 and MDA levels in the lungs were analyzed in Phase 2. RESULTS: Mortality was lower in the ischemic preconditioning group subjected to 90 minutes of ischemia compared to the group without ischemic preconditioning (I-90: 50% and IPC-90: 15%, p=0.018), and it was lower in the ischemic preconditioning group as a whole compared to the groups without ischemic preconditioning (IPC-14% and I=30%, p=0.006). Lower levels of MDA, CINC-1, and CINC-2 were observed in the animals that were subjected to ischemic preconditioning compared to the animals that were not (MDA: I-45=1.23 nmol/mg protein, and IPC-45=0.62 nmol/mg protein, p=0.0333; CINC-1: I-45=0.82 ng/mL and IPC-45=0.67 ng/mL, p=0.041; CINC-2: I-45=0.52 ng/mL and IPC-45=0.35 ng/mL, p=0.032). CONCLUSION: Ischemic preconditioning reduces mortality, inflammatory process and oxidative stress in rats subjected to intestinal ischemia and reperfusion.
Subject(s)
Animals , Male , Inflammation Mediators/metabolism , Ischemic Preconditioning/mortality , Mesenteric Ischemia/metabolism , Oxidative Stress/immunology , Reperfusion Injury/mortality , Chemokine CXCL1/analysis , Chemokines, CXC/analysis , Enzyme-Linked Immunosorbent Assay , Lung/metabolism , Lung/physiopathology , Malondialdehyde/analysis , Mesenteric Arteries/metabolism , Mesenteric Ischemia/mortality , Rats, Wistar , Statistics, NonparametricABSTRACT
O presente trabalho tem como objetivo investigar a influência de materiais utilizados na prática odontológica (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) na resposta inflamatória de fibroblastos cultivados de polpa dental humana de dentes permanentes em relação à expressão e produção de mediadores da inflamação. As culturas primárias de fibroblastos foram estabelecidas a partir do tecido pulpar de terceiros molares hígidos. Após a quarta passagem, os fibroblastos foram estimulados pelos materiais e pelos materiais seguidos por LPS de E. coli pelos tempos de 6 e 24 horas. Os testes utilizados foram: MTT, Trypan Blue, Análise de Griess, PCR quantitativo e ELISA. Os dados foram analisados estatisticamente aplicando-se o teste ANOVA a 1 critério e pós-teste de Tukey e ANOVA a 2 critérios e teste de correção de Bonferroni (p<0,05). Os materiais SB10 (Single Bond 1:100) e DY (Dycal) afetaram a viabilidade celular com diminuição do metabolismo. Os materiais SB1 (Single Bond 1:1.000), SB10 (Single Bond 1:100) e VB (Vitrebond) seguidos de LPS de E. coli diminuíram o metabolismo celular de maneira estatisticamente significativa. Os níveis de óxido nítrico produzidos foram diminuídos quando os fibroblastos foram estimulados pelo KM (Ketac Molar). A expressão gênica para pró-colágeno tipo I foi diminuída quando os fibroblastos foram estimulados pelos materiais SB10 (Single Bond 1:100), SB (Single Bond polimerizado) e DY (Dycal). Para o SDF-1_/CXCL12 houve um aumento da expressão para o grupo estimulado apenas por LPS de E. coli, SB10 (Single Bond 1:100) e DY (Dycal). Para o IL-6 notou-se uma diminuição significativa para o grupo estimulado por H1000 (HEMA 1000 nM) e um aumento para o grupo SB10 (Single Bond 1:100). A expressão gênica de IL- 8/CXCL8 diminuiu para os fibroblastos estimulados pelas três concentrações de HEMA e de Single Bond, VB (Vitrebond) e DY (Dycal) no período de 6 horas e houve um aumento para os materiais SB10 (Single Bond 1:100) e VB...
The aim of the present study is to investigate the influence of dental materials (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) in the inflammatory response of human dental pulp fibroblasts from permanent teeth in relation to inflammatory mediators expression. and production. Primary cultures were established from third molars pulp tissue. After the fourth passage, the fibroblasts were stimulated only by materials and also by the materials followed by LPS from E. coli for 6 and 24 hours. Data were statistically analyzed using Oneway ANOVA and Tukey post-test and Two-way ANOVA followed by Bonferroni post-test (p<0.05). SB10 (Single Bond 1: 100) and DY (Dycal) affected cell viability and consequently decreased cell metabolism. SB1 (Single Bond 1:1,000), SB10 (Single Bond 1:100) and VB (Vitrebond) followed by LPS E. coli decreased cell metabolism. Nitric oxide levels were reduced when fibroblasts were stimulated by KM (Ketac Molar). Pro-collagen type I expression was reduced when fibroblasts were stimulated by SB10 (Single Bond 1:100), SB (polymerized Single Bond) and DY (Dycal). SDF-1_/CXCL12 expression was increased for the group stimulated only by LPS from E. coli, SB10 (Single Bond 1:100) and DY (Dycal). IL-6 expression had a significant decrease in the group stimulated by H1000 (HEMA 1000 nM) and an increase for SB10 (Single Bond 1:100) group. The expression of IL-8/CXCL8 decreased when fibroblasts were stimulated by the three concentrations of HEMA and of Single Bond, VB (Vitrebond) and DY (Dycal) at 6 hours and increased for SB10 (Single Bond 1:100) and VB (Vitrebond) at 24 hours. There was decrease in SDF-1_/CXCL12 production for the three concentrations of HEMA and DY (Dycal) and a declining trend for the other materials tested. The production of IL-6 was increased by VB (Vitrebond) and KM (Ketac Molar). The production of IL-8/CXCL8 increased by SB1 (Single Bond 1:1,000), VB...
Subject(s)
Humans , Fibroblasts , Dental Materials/toxicity , Dental Pulp , Analysis of Variance , Collagen Type I/analysis , Enzyme-Linked Immunosorbent Assay , /analysis , Molar , Matrix Metalloproteinases/analysis , Matrix Metalloproteinases , Polymerase Chain Reaction , Chemokines, CXC/analysis , Chemokines, CXC , Time FactorsABSTRACT
O presente trabalho tem como objetivo investigar a influência de materiais utilizados na prática odontológica (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) na resposta inflamatória de fibroblastos cultivados de polpa dental humana de dentes permanentes em relação à expressão e produção de mediadores da inflamação. As culturas primárias de fibroblastos foram estabelecidas a partir do tecido pulpar de terceiros molares hígidos. Após a quarta passagem, os fibroblastos foram estimulados pelos materiais e pelos materiais seguidos por LPS de E. coli pelos tempos de 6 e 24 horas. Os testes utilizados foram: MTT, Trypan Blue, Análise de Griess, PCR quantitativo e ELISA. Os dados foram analisados estatisticamente aplicando-se o teste ANOVA a 1 critério e pós-teste de Tukey e ANOVA a 2 critérios e teste de correção de Bonferroni (p<0,05). Os materiais SB10 (Single Bond 1:100) e DY (Dycal) afetaram a viabilidade celular com diminuição do metabolismo. Os materiais SB1 (Single Bond 1:1.000), SB10 (Single Bond 1:100) e VB (Vitrebond) seguidos de LPS de E. coli diminuíram o metabolismo celular de maneira estatisticamente significativa. Os níveis de óxido nítrico produzidos foram diminuídos quando os fibroblastos foram estimulados pelo KM (Ketac Molar). A expressão gênica para pró-colágeno tipo I foi diminuída quando os fibroblastos foram estimulados pelos materiais SB10 (Single Bond 1:100), SB (Single Bond polimerizado) e DY (Dycal). Para o SDF-1_/CXCL12 houve um aumento da expressão para o grupo estimulado apenas por LPS de E. coli, SB10 (Single Bond 1:100) e DY (Dycal). Para o IL-6 notou-se uma diminuição significativa para o grupo estimulado por H1000 (HEMA 1000 nM) e um aumento para o grupo SB10 (Single Bond 1:100). A expressão gênica de IL- 8/CXCL8 diminuiu para os fibroblastos estimulados pelas três concentrações de HEMA e de Single Bond, VB (Vitrebond) e DY (Dycal) no período de 6 horas e houve um aumento para os materiais SB10 (Single Bond 1:100) e VB...
The aim of the present study is to investigate the influence of dental materials (Single Bond, HEMA, Vitrebond, Ketac Molar e Dycal) in the inflammatory response of human dental pulp fibroblasts from permanent teeth in relation to inflammatory mediators expression. and production. Primary cultures were established from third molars pulp tissue. After the fourth passage, the fibroblasts were stimulated only by materials and also by the materials followed by LPS from E. coli for 6 and 24 hours. Data were statistically analyzed using Oneway ANOVA and Tukey post-test and Two-way ANOVA followed by Bonferroni post-test (p<0.05). SB10 (Single Bond 1: 100) and DY (Dycal) affected cell viability and consequently decreased cell metabolism. SB1 (Single Bond 1:1,000), SB10 (Single Bond 1:100) and VB (Vitrebond) followed by LPS E. coli decreased cell metabolism. Nitric oxide levels were reduced when fibroblasts were stimulated by KM (Ketac Molar). Pro-collagen type I expression was reduced when fibroblasts were stimulated by SB10 (Single Bond 1:100), SB (polymerized Single Bond) and DY (Dycal). SDF-1_/CXCL12 expression was increased for the group stimulated only by LPS from E. coli, SB10 (Single Bond 1:100) and DY (Dycal). IL-6 expression had a significant decrease in the group stimulated by H1000 (HEMA 1000 nM) and an increase for SB10 (Single Bond 1:100) group. The expression of IL-8/CXCL8 decreased when fibroblasts were stimulated by the three concentrations of HEMA and of Single Bond, VB (Vitrebond) and DY (Dycal) at 6 hours and increased for SB10 (Single Bond 1:100) and VB (Vitrebond) at 24 hours. There was decrease in SDF-1_/CXCL12 production for the three concentrations of HEMA and DY (Dycal) and a declining trend for the other materials tested. The production of IL-6 was increased by VB (Vitrebond) and KM (Ketac Molar). The production of IL-8/CXCL8 increased by SB1 (Single Bond 1:1,000), VB...
Subject(s)
Humans , Fibroblasts , Dental Materials/toxicity , Dental Pulp , Analysis of Variance , Collagen Type I/analysis , Enzyme-Linked Immunosorbent Assay , /analysis , Molar , Matrix Metalloproteinases/analysis , Matrix Metalloproteinases , Polymerase Chain Reaction , Chemokines, CXC/analysis , Chemokines, CXC , Time FactorsABSTRACT
Chemokines recruit and activate leukocytes, assisting granuloma formation. Herein, we evaluated plasma chemokines in patients with active tuberculosis (ATB) and after completing treatment (TTB) and compared them to BCG-vaccinated healthy controls (HC). Levels of chemokines were measured by cytometric bead array. Levels of CXCL8, CXCL9 and CXCL10 were higher in ATB patients compared to HC, but they decreased in TTB. Levels of CCL2 and CCL5 in ATB patients were similar to those observed in HC. Thus, the high levels of CXC-chemokines detected during ATB, which can modulate the trafficking of immune cells from the periphery to the site of infection, were reversed by anti-mycobacterial treatment.